Maria Chatziathanasiadou
Biologist, MSc, PhD
Chemistry Department, University of Ioannina, Greece.

ORCID: 0000-0002-9136-981X

Education

• Bachelor degree: in Biology (2013)
  Department of Biology, University of Patras, Greece
• MSc (2016)
  University of Ioannina, Greece
  Master thesis: “Development of UHPLC-MS/MS combinational methods for the quantitation of anticancer drugs in patient blood and cancer cell lines – ISO 17025:2005 Accreditation”
• PhD student (since 2016)

Honors

• Fellowship award from the General Secretariat for Research and Technology (GSRT) and the Hellenic Foundation for Research and Innovation (HFRI) (Grant Number: 1090)
• Onassis Foundation fellowship for the “2018 Lectures in Biology and Chemistry”, July 16-20, Heraklion, Crete
• First prize for poster presentation entitled: “Investigation of the interactions of silibinin with 2-hydroxypropyl-β-cyclodextrin through biophysical techniques and computational methods” by ChemMedChem, in the «IXth Joint Meeting in Medicinal Chemistry».

Publications

1. Development and validation of simple step protein precipitation UHPLC-MS/MS methods for quantitation of temozolomide in patient plasma samples. Mohamed A. El Mubarak, Evgenios K.Stylos, Maria V. Chatziathanasiadou, Charikleia Danika, G.A.Alexiou, P.Tsekeris, Alexander Renziehausen, Tim Crook, Nelofer Syed, Gregory B.Sivolapenko, Andreas G.Tzakos. J. Pharm. Biomed. Anal. 2018. doi.org/10.1016/j.jpba.2018.09.019.
2. Designing Natural Product Hybrids Bearing Triple Antiplatelet Profile and Evaluating Their Human Plasma Stability. Tsiailanis A, Tsoumani M, Stylos EK, Chatziathanasiadou MV, Kellici TF, Mavromoustakos T, Tselepis AD, Tzakos AG. Methods Mol Biol. 2018;1824:371-385
3. Tailoring acyclovir prodrugs with enhanced antiviral activity: rational design, synthesis, human plasma stability and in vitro evaluation. Chayrov RL, Stylos EK, Chatziathanasiadou MV, Chuchkov KN, Tencheva AI, Kostagianni AD, Milkova TS, Angelova AL, Galabov AS, Shishkov SA, Todorov DG, Tzakos AG, Stankova IG.. Amino Acids. 2018 May 19. doi: 10.1007/s00726-018-2590-y
4. Lipophilic ester and amide derivatives of rosmarinic acid protect cells against H2O2-induced DNA damage and apoptosis: the potential role of intracellular accumulation and labile iron chelation. Gerogianni PS, Chatziathanasiadou MV, Diamantis DA, Tzakos AG, Galaris D. Redox Biol. 2018 May;15:548-556. doi: 10.1016/j.redox.2018.01.014
5. Amplifying and broadening the cytotoxic profile of quercetin in cancer cell lines through bioconjugation. Chatziathanasiadou MV, Geromichalou EG, Sayyad N, Vrettos EI, Katsikoudi A, Stylos E, Bellou S, Geromichalos GD, Tzakos AG. Amino Acids. 2018 Feb; 50(2):279-291. doi: 10.1007/s00726-017-2514-2
6. Enriching the biological space of natural products and charting drug metabolites, through real time biotransformation monitoring: The NMR tube bioreactor. Chatzikonstantinou AV, Chatziathanasiadou MV, Ravera E, Fragai M, Parigi G, Gerothanassis IP, Luchinat C, Stamatis H, Tzakos AG.. Biochim Biophys Acta. 2018 Jan; 1862(1):1-8. doi: 10.1016/j.bbagen.2017.09.021
7. Rational design and structure-activity relationship studies of quercetin-amino acid hybrids targeting the anti-apoptotic protein Bcl-xL. Kellici TF, Chatziathanasiadou MV, Lee MS, Sayyad N, Geromichalou EG, Vrettos EI, Tsiailanis AD, Chi SW, Geromichalos GD, Mavromoustakos T, Tzakos AG. Org Biomol Chem. 2017 Sep 26;15(37):7956-7976
8. Tailoring naringenin conjugates with amplified and triple antiplatelet activity profile: Rational design, synthesis, human plasma stability and in vitro evaluation. Stylos E, Chatziathanasiadou MV, Tsiailanis A, Kellici TF, Tsoumani M, Kostagianni AD, Deligianni M, Tselepis AD, Tzakos AG.. Biochim Biophys Acta. 2017 Nov;1861(11 Pt A):2609-2618
9. Exploring the interactions of irbesartan and irbesartan-2-hydroxypropyl-β-cyclodextrin complex with model membranes. Liossi ΑS, Ntountaniotis D, Kellici TF, Chatziathanasiadou MV, Megariotis G, Mania M, Becker-Baldus J, Kriechbaum M, Krajnc A, Christodoulou E, Glaubitz C, Rappolt M, Amenitsch H, Mali G, Theodorou DN, Valsami G, Pitsikalis M, Iatrou H, Tzakos AG, Mavromoustakos T. Biochim Biophys Acta. 2017 Jun;1859(6):1089-1098
10. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) based bioavailability of the major classes of phytochemicals. Stylos E, Chatziathanasiadou MV, Syriopoulou A, Tzakos AG. J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Mar 15;1047:15-38
11. Functional components of carob: linking the chemical and biological space. Goulas V, Stylos E, Chatziathanasiadou MV, Mavromoustakos T, Tzakos AG. IJMS Int J Mol Sci. 2016 Nov 10;17(11). pii: E1875
12. Mapping the interactions and bioactivity of quercetin-(2-hydroxypropyl)-β-cyclodextrin complex. Kellici TF, Chatziathanasiadou MV, Diamantis D, Chatzikonstantinou AV, Andreadelis I, Christodoulou E, Valsami G, Mavromoustakos T, Tzakos AG. Int J Pharm. 2016 Sep 10;511(1):303-11
13. Investigation of the Interactions of Silibinin with 2-Hydroxypropyl-β-cyclodextrin through Biophysical Techniques and Computational Methods. Kellici TF, Ntountaniotis D, Leonis G, Chatziathanasiadou M, Chatzikonstantinou AV, Becker-Baldus J, Glaubitz C, Tzakos AG, Viras K, Chatzigeorgiou P, Tzimas S, Kefala E, Valsami G, Archontaki H, Papadopoulos MG, Mavromoustakos T. Mol Pharm. 2015 Mar 2;12(3):954-65
14. Expression of the microRNA regulators Drosha, Dicer and Ago2 in non-small cell lung carcinomas. Prodromaki E, Korpetinou A, Giannopoulou E, Vlotinou E, Chatziathanasiadou Μ, Papachristou NI, Scopa CD, Papadaki H, Kalofonos HP, Papachristou DJ. Cell Oncol (Dordr). 2015 Aug;38(4):307-17
15. Direct binding of Bcl-2 family proteins by quercetin triggers its pro-apoptotic activity. Primikyri A, Chatziathanasiadou MV, Karali E, Kostaras E, Mantzaris MD, Hatzimichael E, Shin JS, Chi SW, Briasoulis E, Kolettas E, Gerothanassis IP, Tzakos AG. ACS Chem Biol. 2014 Dec 19;9(12):2737-41

Skills

• Participation in the ISO/IEC 17025:2005 accreditation process of the Laboratory of Organic Chemistry of University of Ioannina. Certification of Training on the implementation requirements of the International Standard ELOT EN ISO / IEC 17025: 2005 for the accreditation of testing laboratories and calibrations
• Certification of Basic Training in Evoq Elite LC-TQ system (ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry), Bruker (Germany)
• Certificate of Training in the operation of Evoq Elite and its software by Bruker Daltonik

Current research

• Study of the anticancer activity of natural products via molecular, biochemical and biophysical techniques (cell culture, SDS-PAGE, western blot, pull down assay, MTT assay, protein purification, isothermal titration calorimetry)
• Quantitation of natural products and drugs in biological samples (blood, cancer cells, tissues) via UHPLC-MS/MS and characterization of their pharmacokinetic parameters
• Study of natural product and drug formulations with cyclodextrins and calixarenes in order to increase their bioavailability via biophysical techniques (Isothermal titration calorimetry, UV-Vis spectroscopy, fluorescence spectroscopy)

The Team

Andreas Tzakos

B.A.C. Director

Associate Professor of Chemical Biology and Bioorganic Chemistry at the University of Ioannina, Greece.

Nikolaos Parisis

Laboratory Manager

Analytical Chemist, MSc.